Introduction:
A Large Volume Pipette Controller is an ergonomic, hand-held electronic device designed for the accurate and safe transfer of liquid volumes, typically ranging from 0.1 ml to 100 ml . It is a fundamental tool in laboratories, used with serological, glass, or plastic pipettes for a wide array of applications, including cell culture, molecular biology, and chemical reagent preparation.
Key Features
Modern pipette controllers come with a host of features designed to enhance usability and precision:
1.Wide Volume Range: They accommodate a broad spectrum of pipettes, from 0.1 ml up to 100 ml .
2.Adjustable Speeds: Many models offer multiple user-selectable aspiration and dispensing speeds, allowing for optimal control when handling liquids of different viscosities. A powerful motor can fill a 25 ml pipette in less than 5 seconds .
3.Rechargeable Battery: Equipped with high-capacity Li-ion batteries, these devices can often operate intermittently for over 8 to 20 hours on a single charge, providing excellent portability and cord-free convenience .
4.Blow-Out Function: A dedicated button or a second stop on the plunger allows for complete ejection of residual liquid from the pipette, which is essential for accuracy, especially with serological pipettes.
Step-by-Step Operating Guide
Here is a general procedure for using a pipette controller:
1. Preparation: Ensure the pipette controller is clean and equipped with a clean, dry hydrophobic filter . Attach a sterile pipette of the appropriate volume by gently inserting it into the nozzle. A secure fit is crucial to prevent leaks.
2. Aspirating Liquid:
Hold the device vertically.
Press the aspiration button to the first stop and hold it.
Immerse the pipette tip 2-4 mm beneath the liquid surface .
Release the button slowly and smoothly to draw the liquid into the pipette. Controlling the release speed is key to avoiding bubbles and ensuring accuracy.
3. Dispensing Liquid:
Place the pipette tip against the inner wall of the receiving vessel at a slight angle.
Slowly press the dispensing button to the first stop to release the main volume. Pause briefly for viscous liquids to allow complete drainage.
For complete delivery, press the button to the second stop or use the dedicated blow-out function to expel any remaining liquid from the tip.
4. Completion: Remove the pipette from the vessel before fully releasing the button to prevent liquid from being drawn back in . Eject the pipette safely if it is disposable.